Journal: Microbial Cell Factories

Article Title: Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth

doi: 10.1186/1475-2859-13-78

Figure Lengend Snippet: Comparison of recombinant LDH activity expressed by strain 090B1, 090B2 and 090B3. After growing while shaking at 200 rpm for 7 h, the cells were collected and crude cell extracts were prepared. The extracts were assayed for LDH activity at pH 6.5 and at the same temperature as that of incubation (among 25°C, 30°C, 34°C, 37°C, 42°C, 45°C or 50°C). The maximum specific LDH activity of each strain was standardized to 100%. The specific LDH activity of their parental B0013-070 was also presented as comparison. The experiments are performed in triplicates. Strain 090B1 showed maximum activity of LDH at 37°C (2.12 U/mg protein), Strain 090B2 yielded activity maximum at 25°C (1.95 U/mg protein), Strain 090B3 showed a activity maximum at 50°C (2.43 U/mg protein). In the parent strain, the highest activity (1.16 U/mg protein) was at 37°C.

Article Snippet: The extracts were assayed for LDH activity using a kit to colorimetrically determine the total bacterial LDH activity (GENMED Scientifics Inc., Arlington, MA, USA) at pH 6.5 and at the same temperature as that of incubation (25°C ~ 50°C).

Techniques: Comparison, Recombinant, Activity Assay, Incubation

Journal: Heliyon

Article Title: A standardized pomegranate fruit extract ameliorates thioacetamide-induced liver fibrosis in rats via AGE-RAGE-ROS signaling

doi: 10.1016/j.heliyon.2023.e14256

Figure Lengend Snippet: Effect of pomegranate fruit extract (PFE, 150 mg/kg/day, orally) on liver/body weight index and liver function parameters in thioacetamide (THIO)-administered rats. Data are presented as means ± SEM, n = 8 /group. *, P < 0.05; **, P < 0.001 vs. control group. # , P < 0.05; ## , P < 0.001 vs. THIO group. ALT, alanine transaminase; AST, aspartate transaminase; ALP, alkaline phosphatase; LDH, lactate dehydrogenase

Article Snippet: Assay kits from Human Diagnostics (Wiesbaden, Germany) were used to assess serum levels of alkaline phosphatase (catalog number 12117, ALP) and lactate dehydrogenase (catalog number 12214, LDH).

Techniques: Control

Journal:

Article Title: Protective Effect of Ischemic Preconditioning on Cold Preservation and Reperfusion Injury Associated With Rat Intestinal Transplantation

doi:

Figure Lengend Snippet: Figure 2. Lactate dehydrogenase (LDH) release (U/mL) as a function of the preservation time. CI: cold ischemia (small bowel extracted and preserved in cold Ringer’s lactate solution at 4°C for a maximum of 4 hours). Prec + CI: preconditioning before the cold ischemia (small bowel clamped for 10 minutes, followed by 5 minutes of reperfusion before preservation in cold Ringer’s lactate solution at 4°C for a maximum of 4 hours). Prec + NAME + CI: preconditioning + NAME (as in preconditioning group but with prior addition of L-NAME). CI + NAME: cold ischemia with previous L-NAME administration. CI + NONOS: cold ischemia with previous NONOS administration. * P < .05 vs. time 0 of the corresponding group. +P < .05 vs. Prec + CI.

Article Snippet: Biochemical Analysis LDH activity in blood and in the preservation solution was determined using a commercial kit purchased from Boehringer Mannheim (Mannheim, Germany).

Techniques: Preserving